Elucidation of the role of the apoptosis inducing DFNA5 protein via model systems in yeast, cell lines and the mouse.

In this project we propose to unravel the role of DFNA5 in apoptosis and to study its function using a variety of approaches. Firstly, we will study the apoptotic mechanism in a yeast model and identify modulators of the apoptotic phenotype and interacting partners of DFNA5. These genes will be further analysed in mammalian cell lines. We will also construct and study a mouse model expressing mutant DFNA5. As a last objective of the project, we aim to crystallize the DFNA5 protein and determine the three dimensional structure by x-ray diffraction.

Keywords:DFNA5, APOPTOSIS, MOLECULAR BIOLOGY

Disciplines:Biochemistry and metabolism, Genetics, Systems biology, Medical biochemistry and metabolism, Molecular and cell biology, Morphological sciences, Oncology

Project type:Collaboration project