Dynamics and high-resolution microscopy of syntenin-2.

Syntenin contains a tandem of PDZ domains. Networks formed via such domains play an important role in cellular signalling. Synt-1 is localised at the plasma membrane and the cytosol. Synt-2, the closest homologue of synt-1, can also be found at the nucleoli and the nuclear speckles. Until now only tetraspanin L6A and phosphatidylinositol 4,5-bisphosphate (PIP2) are known to interact with synt-2. Remarkably synt-2 colokalizes with nuclear PIP2 and the subcellular targeting of synt-2 is dependent on PIP2. Secondly synt-2 shuttles between the plasma membrane and nuclear PIP2 pool during which it can form larger complexes with other proteins. The goal of this project is to study the dynamics of synt-2 in the nucleus using FCS and FRAP. Besides this the codiffusion of possible binding partners like the nucleolar protein of 40kDa will be studied using FCCS.
 

Keywords:Syntenine-2

Disciplines:Biochemistry and metabolism, Systems biology, Medical biochemistry and metabolism

Project type:PhD project