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Dipeptidyl-peptidase 9 in human leucocytes.

This project will focus on the role of dipeptidyl-peptidase 9 (DPP9) in leukocytes. This enzyme is capable of cutting a dipeptide off a peptide after a proline at the penultimate position. However, its physiological role remains elusive. We want to solve the answer to this problem by tackling three key questions. Where exactly do we find DPP9 in leukocytes? It is important to know where in the cell DPP9 resides and if it moves upon cellular activation. Also, do we find equal amounts of DPP9 in different types of leukocytes? Can we identify interaction partners of this large intracellular protein? To determine the function of DPP9, it helps to know with which proteins it interacts. If the function of those proteins is known, it is likely that DPP9 has a function in the same pathway. Can we change cellular functions by changing the expression and/or enzyme activity of DPP9? We can inhibit DPP9 function by either inhibiting its formation or its enzymatic activity. Both methods might lead to (different) effects on the cellular level and indicate in which cellular roles DPP9 is involved. With these three approaches the physiological role of DPP9 in leukocytes might finally be revealed.
Date:1 Oct 2012 →  30 Sep 2014
Disciplines:Biochemistry and metabolism, Immunology, Medical biochemistry and metabolism