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Project
Development of novel expression technology for eukaryotic membrane proteins: application to G-protein coupled receptors
(1) Integration of several engineering steps for the optimalisation of GPCR overexpression in engineered yeast strains with increased ER-membrane capacity and use of an existing (T4 lysozyme insertion) and a new (nanobody complexation by coëxpression) method for GPCR-stabilisation and crystallisation (2) Production, extraction, purification and crystallization of the GPCR membrane proteins
Date:1 Oct 2010 → 30 Sep 2014
Keywords:nanobody, GPCR, yeast engineering
Disciplines:Systems biology, Biomechanical engineering, Other biotechnology, bio-engineering and biosystem engineering, Biomaterials engineering, Industrial biotechnology, Medical biochemistry and metabolism, Other (bio)medical engineering, Environmental engineering and biotechnology, Biological system engineering, Biochemistry and metabolism