< Back to previous page

Project

Development of an imaging system, for videorate superresolution imaging

Fluorescence microscopy is a valued tool in the life and material sciences to visualize and interpret interactions on a micro- and nanoscale level. It has led to many discoveries and is continuously improving in terms of spatial and
temporal resolution, imaging speed of large samples and the amount of information that can be retrieved from raw data. In this work we sought to improve (super-resolution) fluorescence imaging, through the careful design of hardware
in particular, with the goal of keeping the instrumentation accessible for experts and non-experts alike. Throughout the chapters of this dissertation we first discuss two of our devices; the "Circulator", a multiplexing super-resolution imaging module that encodes color in the point-spread-function; the "Resonator", a method for the (semi-) simultaneous visualization of a molecule’s excitation spectrum. We also compare and evaluate scientific and industrial cameras as options for superresolution
SOFI imaging. Finally, we will go over our two microscope designs; the "HIT-SIM", a fast and robust super-resolution SIM microscope; the "TriScan", a diverse system capable of fast sectioned imaging of 3D samples and super-resolution (SMLM) imaging. All of these imaging tools have been developed over the last years with the intent to keep costs low, overall dimensions small and reproducibility and performance high.

Date:12 Jan 2018 →  14 Jun 2023
Keywords:Bio-imaging, Nanobiology
Disciplines:Biochemistry and metabolism, Systems biology, Medical biochemistry and metabolism
Project type:PhD project