DEMONSTRATION AND VALIDATION OF AN INTEGRATED FO-SPR BIOSENSOR FOR CONTINUOUS SENSING IN BIOPROCESSING

The aim of this project is to prove the feasibility of CONSENSE technology towards sustainable bioprocess monitoring and take the next step by providing a proof of concept demonstrator at the systems level. In the project, the cell-based model system will be accessible with dynamic behavior. Real-time biologic tests will be used to generate information on the biology of response dynamics. In this project, I will study the potential concept of a DNA nanoswitch on a FO-SPR platform for continuous protein monitoring, which includes (i) testing internal references on optical fibers to compensate for internal (e.g., fluctuating light sources) and external (e.g., fluctuating temperature) interference; (ii) constructing a demonstrator to integrate sensor probes into the product stream of a cell-based bioreactor for both sample and optical interactions; (iii) modifying the nano-switch concept to detect proteins in the case of the relevant model; (iv) generate a feasibility dataset on all biosensor system solutions for their performance characteristics (stability, sensitivity, specificity, etc.) in the cell culture bioreactor production stream towards benchmark ELISA.