Comparison of nano-injection and aquatic exposure in fish embryo tests: can differences in toxicity be explained by accumulation kinetics?

Nano-injection is a promising alternative exposure route for toxicological research using fish embryos. We recently started applying nano-injections and observed differences in toxicity compared to exposure via water. While it is plausible that uptake and biotransformation play a role in these differences, this has never been investigated. The aim of this project is to characterize and understand differences in toxicity following nano-injection compared to aquatic exposure. We will compare accumulation, biotransformation, molecular events and physiological effects after exposure of zebrafish embryos to the same chemical via both exposure routes. This information is essential to evaluate the applicability of this promising alternative exposure route in (eco)toxicology.

Keywords:ZEBRAFISH, ACCUMULATION, KINETICS, TOXICOLOGY

Disciplines:Animal biology, General biology, Genetics, Veterinary medicine