A compact microscope for long term fluorescence imaging of live cells

The research consortium applies for an automated compact microscope for brightfield and fluorescence imaging of live cell assays. The system includes an incubator for long term imaging (up to weeks) and will contribute to several ongoing projects, namely the quantification of cellular forces using molecular sensors, tracking the biological fate of nanoparticles, the development of fluorescent report assays for organoid cultures and imaging endothelial cell layers under flow. The Lionheart FX system will address the shortage of available microscopes for long-time fluorescence imaging of live-cell assays. We anticipate that researchers from other groups will also benefit from having this compact imaging system at the Nanocenter.

Keywords:Fluorescence microscopy, Live-cell assays, FRET sensors, 3D cell culture systems, Drug delivery systems, Cell migration

Disciplines:Single-cell data analysis, Spectroscopic methods, Cellular interactions and extracellular matrix, Molecular medicine, Medical nanotechnology, Biomaterials, Nanobiotechnology, Molecular and cellular biomechanics