In-capillary drug metabolism study using microsomes and capillary electrophoresis

This project concentrates on investigating and developing a method that allows to look at the concentration of the cofactors NADPH or NADH that are important in Cytochrome P450 reactions. Up until now this was studied with capillary electrophoresis (CE) in several ways: first we applied CE in an off-line mode, looking at the substrate and/or reaction product. Afterwards we concentrated on the cofactor as a generic way to approach metabolic testing. Most recent work deals with an in-line test to look at the cofactor, and these data were obtained with a more simplified enzyme model namely Supersomes. In a next step, this should still be applied on a more true but also more complicated model such as microsomes. The purpose is to first tackle the problem of adsorption of the microsomal material to the inside capillary wall with the use of off-line tests. Then we will develop an in-line model focusing on the cofactor and using microsomes.

Keywords:iteratief lerende controle, evolutionary biology, Avalonia, geodynamics, Machine tools, anisotropy of magnetic susceptibility, Motion control, basin analysis, Arthritis, behavioural ecology, early Palaeozoic, Biomechanics, experimentele identificatie, game theory, Pancreas, Protein Kinase D, niet-lineaire systemen, palaeontology, Immunology, Classification models, Spondyloarthritis, vespidae, cleavage, slate belt, phyllosilicate fabric, social wasps, Signal transduction, Belgium, Critical illness, Mitochondria, Metabolism, Healthcare sector, androgens, Atlas, Nano fibrious material, Electrospinning, Sertoli cel barrier, Extensional Rheology, Biochemistry, social evolution, microbiology, microarray, Sertoli cel, Alzheimer, PhageBiotics, spermatogenesis, conditional knockout, micrarray, androgen receptor, OLP, Ordered Liquid Phases, Emission tomography, pinhole SPECT, microSPECT, image quality, Parenting, Learning problems, Peru, Microsomes, antibacterial, phage therapy

Disciplines:Chemical product design and formulation, Biomaterials engineering