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BASIC: Biomarkers of respiratory Allergy Susceptiblity In Children (R-7536)
Early life exposures can alter DNA methylation patterns, and thereby predispose a child to develop respiratory allergy (RA) later in life. Longitudinal birth cohorts are instrumental to study disease development and the use of saliva, as non-invasive DNA source, has simplified biomarker research. Recently we identified differential methylation gene regions (DMR) in saliva of children with RA vs. controls in 2 independent longitudinal birth cohorts. Illumina Methylation 450K BeadChips revealed 13 DMR in saliva from 11y old allergic children (N=26) vs. controls (N=20). 5 DMR were located in genes involved in IL4 signalling and Th2-response, showing a link with wheezing and other RA phenotypes. The 13 DMR were selected for further biological and technical validation by iPLEX MassArray analysis in the same birth cohort as well as in a second cohort involving 5y old children (N=78). BASIC aims to confirm initial observations and develop added-value for recently identified biomarkers for prediction and/or diagnosis of RA. More specific we aim to: 1) validate these saliva biomarkers in a new birth cohort in the context of preventive screening; and 2) confirm the applicability of the biomarkers in clinically characterized children, including various RA subtypes (e.g. hay fever, house dust allergy). The ultimate goal is to identify objective biomarkers that in the long run could contribute to the development of new prevention strategies, particularly in young people.
Date:1 Jan 2017 → 31 Dec 2019
Keywords:allergy, epigenetics, saliva
Disciplines:Applied mathematics in specific fields, Astronomy and space sciences, Classical physics, Materials physics, Mathematical physics, Quantum physics