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Cell culture media for differentiation of stem cells into hepatocytes

The invention relates to methods and media for inducing or maintaining a mature hepatocyte phenotype in a cell, the method comprising the step of cultivation cells in a cell culture medium comprising at least 10 mg amino acids/ml medium, wherein at least 50 % (w/w) of the concentration of said amino acids in said medium is provided by one up to five amino acids and, with the proviso that said one up to five amino acids is not one of Thr, Tyr, Cys, Met, Arg and His.
Patent Publication Number: WO2018229274
Year filing: 2018
Year approval: 2019
Year publication: 2018
Status: Requested
Technology domains: Biotechnology
Validated for IOF-key: Yes
Attributed to: Associatie KULeuven