Cell culture media for differentiation of stem cells into hepatocytes

The invention relates to methods and media for inducing or maintaining a mature hepatocyte phenotype in a cell, the method comprising the step of cultivation cells in a cell culture medium comprising at least 10 mg amino acids/ml medium, wherein at least 50 % (w/w) of the concentration of said amino acids in said medium is provided by one up to five amino acids and, with the proviso that said one up to five amino acids is not one of Thr, Tyr, Cys, Met, Arg and His.

Patent Publication Number: EP3638772

Year filing: 2020

Year approval: 2021

Year publication: 2020

Status: Requested

Technology domains: Biotechnology

Validated for IOF-key: Yes

Attributed to: Associatie KULeuven