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Infrastructure

A multimodal non-linear optical platform for 2l3-photon excitation and 2ndl3rd harmonic imaging deep inside living tissues and animals (Multimodal NLO imaging platform)

The infrastructure is an advanced and multimodal non-linear optical (NLO) imaging platform for imaging of cells in their native, intact, 3-dimensional (3D) environment over extended periods of time using 2-photon (2P) and 3-photon (3P) excitation principles while providing extra structural context and label-free information by exploiting 2nd and 3rd harmonic signals generated by endogenous molecules, nanomedicines or pollutants.
The platform with its dual 2P|3P pulsed laser configuration:
* allows to reach deeper into tissue structures, enabling non-invasive in-vivo optical recordings in various tissues at 2-3 times the depth achievable with conventional 2P systems
* makes harmonics more easily accessible as they have become redshifted, which readily provide extra context (collagen, myelinization, microtubules, blood flow).
* is an inverted and upright microscope with 3P excitation (at 1300 nm) with high pulse energy and low repetition rate lasers.

Type: Equipment
Location type: Single sited
Accessibility: Everyone
In use: 1 Jul 2023 →  Today
Disciplines: Cancer biology, Developmental neuroscience, Neurophysiology, Nonlinear optics and spectroscopy, Vascular diseases
Keywords: multiphoton excitation, intravital microscopy, seconf and thord harmonic generation, deep infrared imaging, nanoparticle