Mapping airflow dynamics and its immunological correlate on a single cell level in CLAD

One part of the research encompasses the analysis of airflow in chronic lung allograft dysfunction (CLAD), using Computational Flow Dynamics (CFD) modeling. Models can help to link morphologial changes in the lung to the experienced symptoms and health of the patiënt. Models will also allow to study how theoretical changes in tissue elasticity (reflecting the changes observed in COPD and ILD) may affect local flow and pressure during theoritical breathing manoeuvers Secondly, pulmonary single cell/nuclear sequencing will be performed to investigate the altered transcriptome in CLAD lungs. This will identify the pathophysiological mechanisms causing the observed morphological changes, creating insights and providing possibilities for therpeutical interventions.