Multicentre interlaboratory analysis of routine susceptibility testing with a challenge panel of resistant strains

Objectives: To elaborate a new national challenge panel of resistant GNB/GPC strains for the validation of routine antimicrobial susceptibility testing (AST) methods, an interlaboratory evaluation was organized. Methods: Results of 12 well-characterized MDR strains tested by 9 laboratories using local disk diffusion (DD) and automated AST (AUST) methods were compared to the reference broth microdilution. Results: Overall categorical agreements (CA) ranged from 70% to 100% for both DD and AUST and were > 90% for all but one strain for all antibiotics. Conclusions: Our multicenter AST study showed good reproducibility and the panel can be used as national resistant reference strains for routine AST validation.

Publication year:2022

Keywords:A1 Journal article

Accessibility:Open