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Project
Use of fluorescent techniques for cytogenetic characterization of Araceae somatic hybridization products
The aim is to create asymmetric somatic hybrids within the Araceae family, and to visualize (partial) chromosome transfer of the donor to the acceptor genome. Genome fragmentation will be induced through microprotoplasts isolation or UV-irradiation and fusion will ne performed either chemically or electrically. A proper GISH protocol will be designed for cytogenetic testing of the obtained hybrids.
Date:1 Feb 2009 → 31 Jul 2012
Keywords:asymmetric hybrids, GISH, chromosome visualization, protoplast fusion, interspecific, microprotoplasts
Disciplines:Agricultural plant production, Biomaterials engineering, Environmental engineering and biotechnology, Other (bio)medical engineering, Plant biology, Other biotechnology, bio-engineering and biosystem engineering, Industrial biotechnology, Horticultural production, Biological system engineering, Biomechanical engineering