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Project

A multimodal non-linear optical platform for 2|3-photon excitation and 2nd|3rd harmonic imaging deep inside living tissues and animals

Unbiased understanding of cellular networks and circuits, is
only possible by studying them in their native and intact
environment. To achieve this, live 3-dimensional (3D)
imaging is required. However, regular microscopy
techniques do not allow deep penetration, and therefore,
tissue dissection remains necessary to optically reach cells
of interest. However, as redshifted infrared (IR) light
scatters less, two photon (2P) IR imaging can be used to
image at greater depths. To push the boundaries of 2P
imaging even further we propose an advanced multimodal
non-linear imaging platform, consisting of an upright and an
inverted microscope connected at the core by a combined
2P and 3P laser assembly. This platform, which is unique in
Flanders, will enable researchers to image deeper into
tissues, eventually allowing transcranial and transcutaneous
imaging. Additionally, the platform will permit the detection
of other non-linear, label-free observables, i.e. second and
third harmonics, which will be used to provide extra
context. With this platform we will grant new insights into
complex cellular behaviors and also expand the use nonlinear
imaging via the CIC, a KU Leuven imaging core.

Date:1 May 2022 →  Today
Keywords:optical platform, multiphoton excitation, deep infrared imaging, intravital microscopy, second and third harmonic generation, nanoparticle
Disciplines:Biomedical image processing, Neurophysiology, Cancer biology, Developmental neuroscience