Optimalisation of in vitro culture conditions (FTOC and OP9) of T cells from human hematopoietic stem cells for the generation and study of mature T cells

In this project we will further study and optimise the generation of mature CD3+TCRab+ (CD4+ and CD8+) T-cells from human HSC (from postnatal thymus and cord blood) using the in vitro OP9-DL1 culture system. Several methods for the generation of tumor antigen specific T-cells will be tested out. Finally, these tumor antigen specific T-cells will be introduced in a preclinical mouse model.