Fast laser scanning imaging for single nanoparticle analysis

Nanoparticle-based applications require increasingly sensitive screening tools, necessitating single-particle, multi-parameter analysis techniques in the native liquid environment. In this work, we present a laser-scanning camera using acousto-optic deflectors and a single-photon counting module. By scanning a highly focused laser beam (532 nm) across a grid of pixels, and by detecting the associated emitted photons with high sensitivity, fluorescent nanoparticles of 100 nm and below can be imaged at frame rates up to 12 kHz.

Publication year:2022