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Project

Assessment of the bioavailability of phytochemicals by means of the MIVO® device.

In order to be able to exert biological activity, phytochemicals which are administered orally need to be absorbed from the gut and reach the general circulation. In our lab, simulations with a gastrointestinal dialysis model with colon phase (GIDM-colon) are already performed to assess gastrointestinal biotransformation and passive absorption. However, active absorption and efflux mechanisms, causing flow of metabolites from the enterocytes towards the gut lumen, are not taken into account. With the current Small Research Grant we intend to expand our GIDM-colon set-up with the MIVO® device (Multi In Vitro Organ device). This device can be seeded with human intestinal tissue and thus can mimic the intestinal barrier to replicate human intestinal absorption. Since it is a continuous double flow system, it is devoid of some of the drawbacks of classical Caco-2 cell experiments. Chlorogenic acid and rutin, two phenolic phytochemicals that are widely present in food, are selected as model compounds to assess the performance of this new device. Experiments will be performed with these two compounds as such and after addition to faeces samples. Results will be compared to previously obtained results of our GIDM-colon system and to in vitro and in vivo literature data.
Date:1 Apr 2022 →  31 Mar 2023
Keywords:PHYTOCHEMISTRY, POLYPHENOLS, IN VITRO MODEL
Disciplines:Food chemistry, In vitro testing, Phytochemistry, Pharmacokinetics