A method for efficient CRISPR/Cas9 Gene editing in difficult to transform arthropods (CASART)

In this concepTT, we want to capitalize on a unique gene editing method that we developed for the spider mite Tetranychus urticae. Mites - together with ticks, spiders and scorpions - belong to the Chelicerata, the second largest group of animals on the planet, and are notoriously difficult to genetically transform. We will collect data on variations in the formulation composition that might further improve editing efficiency. We will show that our method, in addition to spider mites, can be used in economically important predatory mites but also in some difficult to transform insects. Last but not least, we will provide proof of principle for the creation of knock-ins. Together this information will allow to broaden the claims of a planned patent.