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Project

The role of RABL3 in the regulation of cell proliferation and in early bone develop

Cell proliferation plays a central role during mammalian preimplantation development as it regulates developmental potential and embryo survival. On one side, the rate of cell division during this stage positively correlates with mammalian embryo viability following in vitro fertilization and embryo transfer. On the other side, in case of stressful or unfavorable conditions, the embryo may halt proliferation and development, entering into a protective diapause state. However, our knowledge of how cell division is regulated during preimplantation development remains limited. In this project, we aim to elucidate crucial signaling pathways and vital downstream genes implicated in cell proliferation/quiescence that regulate cell division with a specific focus on the role of Wnt/ß-catenin signaling, for which we have obtained preliminary indications. We will use mouse reporters for cell cycle/quiescence in order to block, stimulate, and knock-out pivotal signal pathways and signaling- associated transcription factors for early development by the CRISPR/Cas9 technology in mouse oocytes/zygotes followed by live imaging. Our results might provide new fundamental insights into the regulation of proliferation and quiescence during mammalian preimplantation development to clarify the missing link between cell signaling and cell proliferation. Consequently, these findings will provide a new model system for in vitro oocytes culture, which is of great interest in the field of reproductive medicine.

Date:15 Nov 2021 →  Today
Keywords:Wnt pathway, Cell cycle regulation, Stem cells, Preimplantation, Embryonic development
Disciplines:Cell signalling, Animal developmental and reproductive biology, Cell growth and development, Stem cell biology
Project type:PhD project