Multigenerational effects modify the tolerance of mosquito larvae to chlorpyrifos but not to a heat spike and do not change their synergism

While interactions with global warming and multigenerational effects are considered crucial to improve risk assessment of pesticides, these have rarely been studied in an integrated way. While heat extremes can magnify pesticide toxicity, no studies tested how their combined effects may transmit to the next generation. We exposed mosquito larvae in a full factorial, two-generation experiment to a heat spike followed by chlorpyrifos exposure. As expected, the heat spike magnified the chlorpyrifos-induced lethal and sublethal effects within both generations. Only when preceded by the heat spike, chlorpyrifos increased mortality and reduced the population growth rate. Moreover, chlorpyrifos-induced reductions in heat tolerance (CTmax), acetylcholinesterase (AChE) activity and development time were further magnified by the heat spike. Notably, when parents were exposed to chlorpyrifos, the chlorpyrifos-induced lethal and sublethal effects in the offspring were smaller, indicating increased tolerance to chlorpyrifos. In contrast, there was no such multigenerational effect for the heat spike. Despite the adaptive multigenerational effect to the pesticide, the synergism with the heat spike was still present in the offspring generation. Generally, our results provide important evidence that short exposure to pulse-like global change stressors can strongly affect organisms within and across generations, and highlight the importance of considering multigenerational effects in risk assessment.

Publication year:2022

Accessibility:Open