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Project

Nanopore sequencing of complete pharmacogenes after CRISPR/Cas9 enrichment in tamoxifen and clopidogrel patients allowing enhanced haplotyping and continuous scale machine learning efficacy prediction.

Pharmacogenetics (PGx) studies how genes influence individual response to drug therapies. Current massively parallel short-read DNA sequencing (SR-MPS) technologies provide limited haplotype information. We want to alleviate this by developing full-gene long-read MPS on PGx genes using an amplification-free CRISPR/Cas9 target enrichment method. Completely-phased genotype data and metadata from 2 cohorts will be used to train a machine learning model.Pharmacogenetics (PGx) studies how genes influence individual response to drug therapies. Current massively parallel short-read DNA sequencing (SR-MPS) technologies provide limited haplotype information. We want to alleviate this by developing full-gene long-read MPS on PGx genes using an amplification-free CRISPR/Cas9 target enrichment method. Completely-phased genotype data and metadata from 2 cohorts will be used to train a machine learning model.

Date:1 Oct 2021 →  Today
Keywords:nanopore sequencing, haplotypng, machine learning, Pharmacogenetics
Disciplines:Pharmogenetics and -genomics