Evaluation and optimisation of a high-throughput sequencing (HTS) based strategy for the detection of viruses and bacteria, in function of certification and in vitro therapy for the creation of virus-free mother plants (VIRVRijUIT)

Viral diseases limit the yield of many important crops. They are particularly problematic in vegetatively propagated crops where they are transmitted from generation to generation. Therefore, viruses are a constant concern for propagation companies from different sectors as they carry a high responsibility for the agricultural and horticultural sector, often with legal and economic consequences. The overall objective of this project is the evaluation and optimisation of a high-throughput sequencing-based strategy for the detection of viruses, in function of certification and in vitro therapy for the creation of virus-free mother plants. Hereby, an adapted HTS strategy will be offered to the different target groups to support them in obtaining virus-free starting material.