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Enhanced enzymatic hydrolysis of cellulose by endoglucanase via expansin pretreatment and the addition of zinc ions

Journal Contribution - Journal Article

One of the major limitations of lignocellulose conversion is the relatively low efficiency of cellulases. Expansins can act as an accessory protein to loosen the rigid cellulose structure and promote cellulose hydrolysis. However, the synergistic action of expansin is not well understood. In this study, we employed quartz crystal microbalance with dissipation to real-time monitor the adsorption of Bacillus subtilis expansin (BsEXLX1) and endoglucanase I (Cel7B) and the hydrolysis of cellulose. The effects of pH, temperature, and zinc ions on the initial adsorption rate and adsorption capacity of BsEXLX1 were examined. When 36.5 mM of zinc ions was added, the irreversible adsorption ratio of BsEXLX1 further increased to 4.63 times the value in the absence of zinc ions, whereas the initial adsorption rate and the hydrolysis rate constants of Cel7B could reach 2.16 times and 2.05 times the values in the absence of zinc ions, respectively.
Journal: Bioresource Technology
ISSN: 0960-8524
Volume: 333
Publication year:2021
BOF-keylabel:yes
IOF-keylabel:yes
BOF-publication weight:10
CSS-citation score:1
Authors:International
Authors from:Higher Education
Accessibility:Open