Analytical flow cytometer for high-throughput microbial phenotypic analysis at single cell level

High throughput flow cytometry performed by an analytical flow cytometer (FC) equipped with an autosampling device, is an important tool in the analysis of microbial populations and communities at the single cell level. The type of measurements and usage frequency require such an apparatus to be located within the building of use. At the Landbouwinstituut of KU Leuven, such an FC apparatus in currently available in two research groups and plays a crucial role in the research of several microbiology research groups. However, its availability is coming under serious pressure, as in one research group the current equipment is old, not perfectly suited for the new intended applications and shows regular mall-functioning and in the other lab, the use of the apparatus is already over-demanded and the machine will in a few years move to another building. This project aims to purchase a novel FC that accommodates all requirements of the existing and intended novel FCM applications at the Landbouwinstituut and to ensure the long-term availability in the building of an FC crucial for high-throughput microbial analysis.

Keywords:Flow cytometer, High throughput, Microbiële gemeenschap, Single cell analyse

Disciplines:Bacteriology, Microbiology not elsewhere classified