< Back to previous page

Publication

Nucleic acid engineering for biosensing applications

Book - Dissertation

Biosensing platforms have become reliable solutions for target detection in a myriad of fields, like environmental monitoring and disease diagnostics. In order to meet the demands of cutting-edge biosensing, protein-based strategies have thus far been the primary focus. However, protein-based biosensing strategies are limited by several hurdles, including sensitivity to changes in the environment (e.g. pH, temperature), batch-to-batch variability, as well as the long, resource-intensive process required for generating and testing new molecules. Interestingly, the inherent stability of NAs in diverse environmental conditions, and the flexibility and cost-effectiveness of their design and prototyping provide a promising alternative approach. In this project, different NA engineering concepts are explored for developing robust, sensitive, and specific biosensing solutions for disease diagnostics, with the main focus on utilization of RNA-cleaving DNA-based enzymes (NAzymes) in concert with other platforms and approaches.
Publication year:2020
Accessibility:Closed