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Project
Improving multiplex digital PCR based nucleic acid quantification using data driven analysis tools
Digital PCR allows higher order multiplexing compared to real time PCR, enabling simultaneous quantification of multiple targets for single cell research and for diagnosis of cancer mutations or infectious agents. These applications require an appropriate data analysis pipeline. We will tackle this unexplored field by designing 16-plex reactions for single cell analysis of macrophages and mutation detection in cancer.
Date:1 Oct 2020 → Today
Keywords:macrophages, multiplex PCR, mutation detection, Digital Polymerase Chain Reaction (dPCR), single cell gene expression, cancer
Disciplines:Cancer diagnosis, Statistics, Molecular diagnostics, Veterinary medicine not elsewhere classified, Biostatistics