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Project
Molecular architecture and assembly of heteromeric membrane proteins.
The surface expression of plasma membrane proteins will be assessed using HA-tags on their extracellular segments, and the total amount using GFP-tagged versions. Heteromeric co-assembly will be tested using FRET between CFP and YFP tagged proteins. Internalization and endosomal recycling will be assessed using photoactivatable and pH sensitive fluorophores.
Date:1 Jan 2015 → 31 Dec 2017
Keywords:PROTEINS
Disciplines:Biochemistry and metabolism, Systems biology, Medical biochemistry and metabolism