Analytical separations of complex samples using novel affinity matrices based on molecularly imprinted polymers (R-2895)

Molecularly Imprinted Polymers (MIPs) are synthetic receptors, which can bind target molecules with a high selectivity and affinity. The general concept of molecular imprinting is based on a polymerization of functional monomers and cross-linkers, surrounding a target molecule. The aim of this project is the development of novel MIPs and their direct integration into analytical separation methods. The miniaturization, which can be achieved in this manner, will allow for the study of limitedly available samples with a complex composition. MIPs can be introduced into separation columns in a variety of manners. Straightforward is the use of crushed monoliths or microspheres, which can be packed into columns. Alternatively, monolith-filled columns can be utilized. In addition to these existing procedures, a new method will be developed by preparing MIPs in situ at the interior walls of capillary columns using immobilized initiators. The developed separation MIPs will be utilized to tackle complex separation problems. The first example focuses on the selective isolation of melphalan modified nucleosides using MIPs prepared via substructure imprinting. To this end, template analogues will be utilized. Using a similar approach, a biotin substructure imprinted MIP will be developed, which can bind biotin labeled biomolecules under non-aqueous conditions.

Keywords:POLYMER NETWORKS

Disciplines:Chemical sciences