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Project

Identification of novel targets and pathways underlying visceral hypersensitivity

 Visceral hypersensitivity (VHS) leading to chronic abdominal pain is the main pathophysiological mechanism underlying irritable bowel syndrome (IBS). However, the pathways involved in VHS remain poorly understood. In this project, I aim to decipher novel molecular mechanisms underlying VHS using a state-of-the-art technique, i.e. single-cell RNA sequencing (scRNA-seq). I will use this technique to investigate and compare the transcriptome of dorsal root ganglion neurons collected from VHS and normosensitive mice. I will employ bioinformatics tools to discover key transcription factors and novel pathways that underlie the mechanisms involved in VHS. In the second part of my project, I will investigate the functional role of the novel targets/pathways previously identified. To this end, I will use gene therapy using engineered viral vectors to modify the gene expression of molecular targets identified by scRNA-seq in sensory neurons in vitro and in vivo. This will allow me to evaluate the functional role of key players in neuronal physiology and the development of VHS. The results of this project will enhance our understanding of visceral pain signaling and will identify novel targets for the development of more effective approaches for the treatment of visceral pain.

Date:1 Oct 2019 →  16 Jun 2022
Keywords:Irritable Bowel Syndrome, single cell RNA sequencing, Visceral hypersensitivity
Disciplines:Other biological sciences not elsewhere classified, Medical systems biology not elsewhere classified