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Publication

Structural Characterization of Monomeric/Dimeric State of p59(fyn) SH2 Domain

Journal Contribution - Journal Article

Src homology 2 (SH2) domains are key modulators in various signaling pathways allowing the recognition of phosphotyrosine sites of different proteins. Despite the fact that SH2 domains acquire their biological functions in a monomeric state, a multitude of reports have shown their tendency to dimerize. Here, we provide a technical description on how to isolate and characterize by gel filtration, circular dichroism (CD), and nuclear magnetic resonance (NMR) each conformational state of p59(fyn) SH2 domain.

Journal: Methods in Molecular Biology: In Silico Methods for Predicting Drug Toxicity
Volume: 1555
Pages: 257-267
Publication year:2017
Keywords:Circular dichroism (CD), Gel filtration chromatography, Monomer/dimer, Nuclear magnetic resonance (NMR), SH2 domain
Accessibility:Closed