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Publication
Research methods
Book Contribution - Chapter
Venoms are complex concoctions consisting of peptides, proteins, salts, and other compounds. As such, they present a unique set of challenges for the venom researcher. This chapter provides a broad overview of the techniques useful in venom research, ranging from gross characterization of the venom's protein content to purification and characterization of individual toxins, bioactivity testing, and evolutionary assessment of toxin-encoding genes. Special attention is paid to unique challenges posed by reptile venom or techniques that have proved to be particularly useful.
Book: Venomous Reptiles: Evolution, Pathophysiology and Biodiscovery
Pages: 153-215
ISBN:978-0-19-930939-9
Publication year:2015
Keywords:Venom, Reptiles, proteins, proteomics, transcriptomics, phylogenetics