AUSEM: AUtomatic freeze-Substitution for advanced Electron Microscopy techniques (AUSEM)

There are different ways to achieve correlated light and electron microscopy (CLEM), where the exact same region of a biological sample is imaged with both types of microscopy. The CLEM methodology that we want to implement is ‘in resin GFP’, in which fluorescent signals are preserved throughout EM sample processing with high pressure freezing and automatic freeze- ubstitution.