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Researcher

Angela Privat Maldonado

  • Research Expertise:Application of low-temperature plasmas for cancer research using 3-dimensional in vitro models and in ovo TUM-CAM model Study of the effect of plasma-generated reactive species in the tumor microenvironment, pancreatic cancer cells and stellate cells, with specific emphasis on the role of stellate cells in the migration of cancer cells upon treatment Live imaging of 3D spheroids, assessment of viability, cell death. Analysis of proliferative markers, extracellular matrix components, hypoxia by immunohistochemistry / immunofluorescence Multi-arrays for 3~D spheroids in paraffin, cryosectioning
  • Keywords:IMMUNOHISTOCHEMISTRY, CANCER RESEARCH, 3D SPHEROID MODEL, ANTICANCER AGENTS, LIVE CELL IMAGING, IN VITRO CULTURE, ATMOSPHERIC PLASMA, MICROSCOPY (FLUORESCENCE), CHICKEN CHORIOALLANTOIC MEMBRANE MODEL, Chemistry (incl. biochemistry)
  • Disciplines:Physics of (fusion) plasmas and electric discharges, Bacteriology, Cell movement, Cell signalling, Cellular interactions and extracellular matrix
  • Research techniques:• Cell culture • Light & fluorescence microscopy • 3D in vitro tumor models • Evaluation of vaccine candidates in vitro • IHC/IF, tissue processing • Method development • In ovo TUM-CAM model • Microbiological techniques
  • Users of research expertise:Any group willing to work with 3D spheroids in vitro, TUM-CAM model for angiogenesis and cancer research, use of low-temperature plasmas for biomedical purposes