< Back to previous page

Researcher

Dorien Verdoodt

  • Research Expertise:Breeding and handling of transgenic animal models for hereditary hearing loss and vestibular dysfunction. Performing auditory brainstem response and distortion oto-acoustic emission measurements to assess hearing function in rodents. Investigating balance through measurement of the vestibular ocular reflex and observation of reflexes and behavior. Noise exposure in mice. Injection of molecules into the inner ear using the posterior semicircular canal approach in adult mice. Development of gene therapy to tackle hearing and vestibular loss in DFNA9 patients using a new humanized DFNA9 mouse model. Experience in immunohistochemistry (cryosections and whole mount) of the Organ of Corti and the spiral ligament to visualize different cell types, inner ear proteins and inner ear inflammation using specific antibodies. Molecular biology techniques involving RNA/DNA isolation, PCR, sequencing, ....
  • Keywords:DEAFNESS, GENE THERAPY, DFNA9, OTOLARYNGOLOGY, Medicine
  • Disciplines:Anaesthesiology, Bioinformatics and computational biology, Biomechanics, Biophysics, Cardiac and vascular medicine, Dermatology, Diagnostics, Endocrinology and metabolic diseases, Gastro-enterology and hepatology, Gerontology and geriatrics, Gynaecology and obstetrics, Hematology, Immunology, Intensive care and emergency medicine, Laboratory medicine, Medical biochemistry and metabolism, Medical imaging and therapy, Medical systems biology, Microbiology, Molecular and cell biology, Morphological sciences, Neurosciences, Oncology, Ophthalmology and optometry, Orthopaedics, Otorhinolaryngology, Palliative care and end-of-life care, Paediatrics and neonatology, Physiology, Psychiatry and psychotherapy, Regenerative medicine, Respiratory medicine, Surgery, Tropical medicine, Urology and nephrology, Other clinical sciences, Forensic medicine, Nursing, Nutrition and dietetics, Speech, language and hearing sciences, Other paramedical sciences, Other medical and health sciences
  • Research techniques:Measuring hearing function in rodents using Tucker-Davis Technologies to perform auditory brainstem responses and distortion oto-acoustic emissions. Assessing inner ear inflammation by performing immunohistochemistry of the organ of Corti and the spiral ligament and staining the immune cells using specific antibodies. Injection of molecules into the inner ear via the posterior semicircular canal approach using the Nanoliter 2020 device. Molecular techniques involving RNA/DNA isolation, PCR and sequencing.
  • Users of research expertise:Researchers that are interested in the generation and phenotyping of animal models for hereditary hearing loss and vestibular failure. Research into the inflammatory processes in the inner ear of mice.