Assessment of skeletal endpoints and exposure in an extended Zebrafish Developmental Toxicity Assay (ZEDTA) using mammalian teratogens.

The number of morphological endpoints in the ZEDTA is much more limited compared to the in vivo developmental toxicity studies. A survey within the laboratories of the proposers pointed out that especially the skeletal endpoints are limited in the ZEDTA. A literature review showed false negative results in the ZEDTA for compounds that showed skeletal abnormalities in mammalian studies. The aim of this project is to assess whether extension of the morphological parameters and evaluation period in the ZEDTA after exposure to mammalian skeletal teratogens during organogenesis increases the concordance of the ZEDTA with the mammalian in vivo developmental toxicity studies.

Keywords:ZEBRAFISH, DEVELOPMENT, NON-ANIMAL TESTING, SKELETAL DISORDERS

Disciplines:Animal biology, Genetics, Biomarker discovery and evaluation, Drug discovery and development, Medicinal products, Pharmaceutics, Pharmacognosy and phytochemistry, Pharmacology, Pharmacotherapy, Toxicology and toxinology, Other pharmaceutical sciences, Veterinary medicine