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Project
Chromatographic Organic Trace Analysis.
A potentiometric sensor is made for the specific detection of an oligonucleotide. The mutation which causes mucoviscidosis is taken as an example. The potentiometric sensor has a PVC/plasticizer rubber coating on a metal conductor. The PVC is aminated, and the oligonucleotide is coupled to the surface via UV irradiation. We then study the interaction of match and mismatch oligonucleotides with the sensor. The sensor should respond selectively to the target oligonucleotide. We then study the possibility to use the sensor in real-time PCR experiments.
Date:3 May 2010 → 2 Mar 2011
Keywords:CHROMATOGRAPHY
Disciplines:Inorganic chemistry, Organic chemistry, Theoretical and computational chemistry, Other chemical sciences