An analytical pipeline for microbial proteins: from peptide to multi-protein complexes in single cells and populations

Research at the Centre of Microbial and Plant Genetics (CMPG) and at the Technology Cluster Bioengineering Technology (CBeT; Technology Campus De Nayer) aims at understanding molecular mechanisms in individual microbes, interplay between microbes, and interactions with higher eukaryotic hosts (human/mammals, plants, insects, #) with the aim of applying this knowledge to improve biotechnological processes. A central activity amongst all CMPG and CBeT groups is the fundamental study and application of proteins from bacteria, fungi and yeasts in various processes such as antibiotic-mediated killing, biosynthesis of bioactive secondary metabolites, ethanol tolerance/production, yeast memory and microbial ecology. Equipment for peptide/protein detection and quantification, peptide/protein purification, and high-throughput single-cell protein analysis is state-of-the-art in modern microbiological labs. This equipment will be used by the different partners as pipeline for protein discovery and analysis, as well as for unraveling signaling pathways induced in single cells.

Keywords:analytical pipeline

Disciplines:Analytical chemistry, Macromolecular and materials chemistry