Automated real-time quantitative live cell analysis

The IncuCyte system is a flexible and automated imaging system that enables real-time quantification of multiple cell behavior parameters over long periods of time, without having to end the assay to take endpoint reads. It thus allows to follow in real-time, through image-based quantitative analysis of multiple cellular parameters: (i) the processes of cell proliferation or cell death, for instance induced by virus infection; (ii) the effect of inhibitors of these processes (i.e. anticancer and antiviral agents); or (iii) the outcome of specific gene knock-down. In this way it provides a significant advantage over typical end-point measurements that do not provide information on kinetics and usually do not allow multiplexing of different cellular parameter observations (such as proliferation, viability, apoptosis induction, virus-induced cytopathogenic effect) on the exact same cell cultures. We will implement this new technology in our studies of functional genome mutations and virus infection on cell behavior, as well as in our drug discovery research to gather faster and more complete insights into the mechanism of action of potential anticancer or antiviral drugs, or the replicative behavior of a drug-resistant cell line or virus.

Keywords:cell analysis

Disciplines:Morphological sciences, Oncology