Towards modelling the human colon microbiota dynamics: an applied systems biology approach

In spite of the importance of the gastrointestinal microbiota in health and disease, interactions between key groups of colon microorganisms are underexplored. Quantitative data, enabling dynamic model development, is currently lacking. The present project proposes to explore the dynamics of a simplified human intestinal microbiota in vitro and in silico.

Large-intestinal hydrogen production affects both colon ecosystem stability and host health. Through a stepwise approach, including mono-, bi-, and triculture fermentations, a microbial community of four key species involved in hydrogen metabolism will be set up in vitro (chemostat). Exploration and quantification of species interactions through monitoring of growth and metabolite production will increase understanding of hydrogen metabolism dynamics. Results will be translated in an in silico community model.