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Publication

Standardization of real-time PCR gene expression data from independent biological replicates

Journal Contribution - Journal Editorial

Gene expression analysis by quantitative reverse transcription PCR (qRT-PCR) allows accurate quantifications of messenger RNA (mRNA) levels over different samples. Corrective methods for different steps in the qRT-PCR reaction have been reported; however, statistical analysis and presentation of substantially variable biological repeats present problems and are often not meaningful, for example, in a biological system such as mouse embryonic stem cell differentiation. Based on a series of sequential corrections, including log transformation, mean centering, and autoscaling, we describe a robust and powerful standardization method that can be used on highly variable data sets to draw statistically reliable conclusions.
Journal: ANALYTICAL BIOCHEMISTRY
ISSN: 0003-2697
Issue: 1
Volume: 379
Pages: 127 - 129
Publication year:2008
Accessibility:Closed