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Project
Prognostic and therapeutic value of gene expression profiles in renal cancer
MicroRNA (miRNA) profiles fo primary human renal cell carcinoma tissue will be linked with clinical outcome data and pathological features to define differentially expressed miRNA clusters, that could serve as prognostic marker and predictor of therapy response.
In a first, restrospective phase we will work on the identification of dysregulated miRNA's, linked with disease aggressiveness and therapy response by whole genome real-time polymerase chain reaction quantification and validate the databy in situ hybridization staining (ISH) on the original formalin fixed paraffin embedded tumoural tissue, so introducing a large scale applicable technique for clinically relevant microRNA detection in the clinical setting.
In a second phase we will use miRNA profiles of nephrectomy-specimens to guide individualized first line adjuvant therapy in a prospective clinical trial.
In a first, restrospective phase we will work on the identification of dysregulated miRNA's, linked with disease aggressiveness and therapy response by whole genome real-time polymerase chain reaction quantification and validate the databy in situ hybridization staining (ISH) on the original formalin fixed paraffin embedded tumoural tissue, so introducing a large scale applicable technique for clinically relevant microRNA detection in the clinical setting.
In a second phase we will use miRNA profiles of nephrectomy-specimens to guide individualized first line adjuvant therapy in a prospective clinical trial.
Date:1 Aug 2009 → 31 May 2018
Keywords:Renal cell carcinoma, Microrna, Predictive biomarker
Disciplines:Laboratory medicine, Palliative care and end-of-life care, Regenerative medicine, Other basic sciences, Other health sciences, Nursing, Other paramedical sciences, Other translational sciences, Other medical and health sciences, Genetics, Systems biology, Molecular and cell biology
Project type:PhD project