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Patent
Screening and sorting of single cells
In general the present invention concerns 1) single cell trapping of a viable cell in separate well from a plurality of wells in an array of wells, 2) single cell analysis for the selected cell and 3) single cell lifting of the yet viable cell from the well by an optical tweezer. Furthermore resent invention concerns a cell trap and lift device for B lymphocytes, the device comprising an array of wells in in polymer matrix comprising an off-stoichiometry thiol-ene polymer of the group consisting of off- stoichiometry thiol-ene (OSTE) and off- stoichiometry thiol-ene-epoxy (OSTE+) or a combination thereof that have been grafted with methacrylated polyethylene glycol (methoxy polyethylene glycol methacrylate or (M-PEG-M)) of a number average molecular weight of Mn 2000. It furthermore concerns using the B lymphocyte trap and lift device for trapping single B lymphocyte cells in wells of the device of present invention and lifting said cell from the cell trapping well by optical tweezers, preferably single beam tweezers.
Patent Publication Number: EP3877085
Year filing: 2021
Year approval: 2022
Year publication: 2021
Status: Requested
URI: link to Espacenet
Technology domains: Measurement, Biotechnology, Chemical engineering
Validated for IOF-key: Yes
Attributed to: Associatie KULeuven