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Profiling activity of cellular kinases in migrating T-cells

Book Contribution - Chapter

T-Lymphocyte kinases are important checkpoints that control T-cell motility by regulating a diverse range of signal transduction pathways. The distinct configuration of kinase events in T-cell could be used to fingerprint the status of T-cells. However, only small fraction human kinases have been characterized so far and little is known about the dynamics of the kinome in motile T-cells. Although several direct and indirect strategies exist to characterize cellular kinase activities, such as RNA interference, antibody arrays, enzyme kinetics, and mass spectrometry, this chapter focuses on an alternative multiplex phosphopeptide array-based methodology, which allows the kinome-wide identification of hyper-activated kinases involved in the regulation of T-cell migration.
Book: T-cell motility: methods and protocols
Series: Methods in Molecular Biology
Pages: 99 - 113
ISBN:9781493990368
Publication year:2019
Accessibility:Closed